About

We designed a gRNA design tool that allows users to generate activation and suppression guides for nuclease inactivated dCasMINI against any gene in the human genome (Lopp & Yeo et al., 2023). We anticipate that our tool will facilitate epigenetic editing studies involving dCasMINI by computing genome-wide activation/suppression guides that can be queried by users based on gene IDs. dCasMINI is a fraction of the size of other Cas molecules like dCas9 (Xu et al., 2021), facilitating single vector packaging across both in vivo and ex vivo delivery modalities (i.e. AAV, LNP, lentivirus/retrovirus), and has a favorable off-target profile, resulting in a reduction of undesired gene targeting events. (Xin et al., 2022)